The right choice for your laboratory!<\/span><\/p>\n[\/vc_column_text][\/vc_column][vc_column width=”1\/4″][vc_progress_bar values=”%5B%7B%22label%22%3A%22Competitor%22%2C%22value%22%3A%2292%22%2C%22color%22%3A%22custom%22%2C%22customcolor%22%3A%22%23ffcc00%22%2C%22customtxtcolor%22%3A%22%23000000%22%7D%2C%7B%22label%22%3A%22SERVA%20Trypsin%22%2C%22value%22%3A%22103%22%2C%22color%22%3A%22bar_blue%22%7D%2C%7B%22label%22%3A%22Competitor%22%2C%22value%22%3A%2294%22%2C%22color%22%3A%22custom%22%2C%22customcolor%22%3A%22%23dd3333%22%2C%22customtxtcolor%22%3A%22%23ffffff%22%7D%5D”][vc_column_text]Protein groups[\/vc_column_text][\/vc_column][vc_column width=”1\/4″][vc_progress_bar values=”%5B%7B%22label%22%3A%22Competitor%22%2C%22value%22%3A%22257%22%2C%22color%22%3A%22custom%22%2C%22customcolor%22%3A%22%23ffcc00%22%2C%22customtxtcolor%22%3A%22%23000000%22%7D%2C%7B%22label%22%3A%22SERVA%20Trypsin%22%2C%22value%22%3A%22281%22%2C%22color%22%3A%22bar_blue%22%7D%2C%7B%22label%22%3A%22Competitor%22%2C%22value%22%3A%22265%22%2C%22color%22%3A%22custom%22%2C%22customcolor%22%3A%22%23dd3333%22%2C%22customtxtcolor%22%3A%22%23ffffff%22%7D%5D”][vc_column_text]Proteins[\/vc_column_text][\/vc_column][\/vc_row][vc_row][vc_column][vc_text_separator title=”The SERVA qualities” color=”blue”][vc_tta_tabs][vc_tta_section title=”Trypsin Sequencing Grade” tab_id=”1499678799530-12eecf50-adf4″][vc_column_text]<\/p>\n
Trypsin Sequencing Grade, modified, from porcine pancreas<\/h1>\n
[\/vc_column_text][vc_column_text]Use this quality for protein digestion for following mass spec analysis. Additionally, you can use this quality for protein sequencing.<\/p>\n
The Lysine residues of this quality were modified by reductive methylation and purified by chromatography afterwards. This results in a stabilization of the Lysine residues against autolysis and thus no Chymotrypsin activity is detectable.\u00a0We test the specifity by degrading the oxidized B-chain of the Insulin.[\/vc_column_text][vc_btn title=”SERVA Shop” shape=”square” color=”blue” link=”url:http%3A%2F%2Fwww.serva.de%2FenDE%2FProductDetails%2F3185_37283_Trypsin_NB_Sequencing_Grade_modified_from_porcine_pancreas_0_0.html|title:Trypsin%20Sequencing%20Grade%20from%20SERVA|target:%20_blank|”][\/vc_tta_section][vc_tta_section title=”Trypsin MS approved” tab_id=”1499678689587-5d8748c8-0d8a”][vc_column_text]<\/p>\n
Trypsin MS approved<\/h2>\n
[\/vc_column_text][vc_column_text]Use this quality for use in mass spectrometry analysis.<\/p>\n
The Lysine residues of this quality were modified by reductive methylation and purified by chromatography\u00a0afterwards. This results in a stabilization of the Lysine residues against autolysis and thus no Chymotrypsin activity is detectable.<\/p>\n
MS approved means that each lot is tested by mass spectrometry.[\/vc_column_text][vc_btn title=”SERVA Shop” shape=”square” color=”blue” link=”url:http%3A%2F%2Fwww.serva.de%2FenDE%2FProductDetails%2F5763_37286_Trypsin_MS_approved_0_0.html|title:Trypsin%20MS%20approved%20from%20SERVA|target:%20_blank|”][\/vc_tta_section][vc_tta_section title=”Trypsin for Cell Culture” tab_id=”1499685811135-98848422-979b”][vc_column_text]<\/p>\n
Trypsin 1:250 from porcine pancreas<\/h1>\n
[\/vc_column_text][vc_column_text]The Serine protease mixture for cell culture contains Chymotrypsin- and non-proteolytic activities. Therefore, Trypsin 1:250 is a special enzyme mixture to achieve maximum cell viability with gentle detachment.<\/p>\n
Use a working concentration of 250 mg\/100ml, pH 7-8.[\/vc_column_text][vc_btn title=”SERVA Shop” shape=”square” color=”blue” link=”url:https%3A%2F%2Fwww.serva.de%2FenDE%2FProductDetails%2F6535_37292_Trypsin_1_to_250_from_porcine_pancreas_lyoph_0_0.html|title:Trypsin%201%3A250|target:%20_blank|”][\/vc_tta_section][\/vc_tta_tabs][\/vc_column][\/vc_row][vc_row][vc_column][vc_text_separator title=”References” color=”blue”][vc_column_text]Customers’ opinion regarding our Trypsin[\/vc_column_text][vc_column_text]\u201eFor several years we have been looking for a real alternative to the \u201esequenzing grade\u201c Trypsin of a well-known manufacturer. We found it:\u00a0Trypsin MS approved<\/em><\/strong>\u00a0by SERVA. It works at least as efficiently and specifically as the \u201egold standard\u201c we used before, and does not generate any significant autolytic peaks. A major plus, particularly for a mass spec core facility, is the moderate pricing.\u201c\u00a0Dr. Stefan Baumeister, Philipps-Universit\u00e4t Marburg<\/strong><\/p>\n“We use Trypsin Premium Grade, MS approved<\/strong>, from porcine pancreas for enzymatic protein digestion. The enzyme is characterized by stable activity, purity and durability. Furthermore, the trypsin is easy to handle, requires very small amounts for the digestion, it dissolves efficiently in buffer and we hardly observe self-digestion.<\/span>”\u00a0Christiane Rewerts, Core Unit Proteomics, Max-Planck-Institut f\u00fcr Psychiatrie M\u00fcnchen<\/strong><\/p>\n\u201eIn the Proteomics Core Facility, we use SERVA trypsin for sample preparation by in-gel digestion for mass spectrometry. The SERVA Trypsin convinces with its efficiency and specificity with a very low miscleavage rate.<\/span>”\u00a0Dr. Andreas Marquardt, Universit\u00e4t Konstanz<\/strong>[\/vc_column_text][vc_column_text]The following institutions already use our Trypsin[\/vc_column_text][vc_single_image image=”228″ img_size=”full” css_animation=”none”][\/vc_column][\/vc_row]<\/p>\n","protected":false},"excerpt":{"rendered":"[vc_row][vc_column][vc_column_text] SERVA Trypsin\u00a0 – The best choice for your lab [\/vc_column_text][vc_text_separator title=”Trypsin from SERVA” color=”blue”][vc_row_inner][vc_column_inner width=”1\/2″][vc_column_text]With\u00a0SERVA\u2018s Trypsin portfolio, you get a coordinated product for many applications. Inform yourself about our qualities for cell culture, mass spectrometry or sequencing.[\/vc_column_text][\/vc_column_inner][vc_column_inner width=”1\/2″][vc_column_text]Diagram: HeLa and E.coli FASP digestion using SERVA Trypsin in comparison with Trypsins of other suppliers. Analysis…<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"yst_prominent_words":[2209,2203,2200,2207,2193,2202,2199,2205,2195,2197,2201,2208,2194,2204,2206,2192,2196,2190,2191,2198],"class_list":["post-290","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/pages\/290","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/comments?post=290"}],"version-history":[{"count":90,"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/pages\/290\/revisions"}],"predecessor-version":[{"id":3190,"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/pages\/290\/revisions\/3190"}],"wp:attachment":[{"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/media?parent=290"}],"wp:term":[{"taxonomy":"yst_prominent_words","embeddable":true,"href":"https:\/\/info.serva.de\/en\/wp-json\/wp\/v2\/yst_prominent_words?post=290"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}