Laemmli buffer for SDS PAGE

Laemmli running buffer for SDS PAGE (10X)

0,25 M TRIS

1,92 M Glycine

1 % SDS

  • For one liter 10X Laemmli running buffer, weigh 30,275 g TRIS, 144,13 g Glycine and 10 g SDS.
  • Transfer all into a 1L beaker.
  • Fill the beaker with 900 ml deionised water and stirr until the chemicals are completely solved.

Laemmli sample buffer for SDS PAGE (2X)

126 mM TRIS/HCl (pH 6,8)

20% Glycerol

4 % SDS

0,02 % Bromophenol Blue

  • Prepare a stock solution 1M TRIS/HCl (pH 6,8) first. Therefore, weigh 121,1 g TRIS, transfer it to a 1L beaker and solve it with 800 ml deionised water. Adjust the pH value to 6,8 with concentrated hydrochloric acid and fill up to one liter with deionised water.
  • Then fill the beaker up to the 1L mark with deionised water.
  • Give it another short stirr and fill it into a screw cap bottle.
  • For 100 ml 2X Laemmli sample buffer fill 12,6 ml of the 1M TRIS/HCl (pH 6,8) stock, 20 g Glycerol, 4 g SDS und 20 mg Bromophenol Blue into a beaker, fill up to 80 ml with deionised water and stirr until the chemicals are completely solved.
  • Then fill the beaker up to the 100ml mark with deionised water.
  • Give it another short stirr and aliquot.
  • Mix 100ml Laemmli running buffer 10X with 900ml deionised water.
  • Fill your SDS PAGE chamber with it.
  • Mix Laemmli running buffer 2X with the same amount of sample.
  • Denature sample 5 minutes at 95°C.
  • Load sample on gel.

Laemmli buffer 10x, for SDS-PAGE is also available from us…

Laemmli sample buffer 2x, for SDS PAGE is also available from us…