SERVA Trypsin  – The best choice for your lab

 

Trypsin from SERVA

With SERVA‘s Trypsin portfolio, you get a coordinated product for many applications. Inform yourself about our qualities for cell culture, mass spectrometry or sequencing.

Diagram: HeLa and E.coli FASP digestion using SERVA Trypsin in comparison with Trypsins of other suppliers. Analysis with LC-MS.

Trypsin has many uses in the laboratory. For example, adherent cells dissolve by use from culture dishes (cell culture). In addition, it is the enzyme of choice in proteomics. Here, proteins are digested to receive peptides in order to analyze them by mass spectrometry (in-gel digestion).

The right choice for your laboratory!

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SERVA Trypsin
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Protein groups

Competitor
SERVA Trypsin
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Proteins

The SERVA qualities

Trypsin NB Sequencing Grade, modified, from porcine pancreas

 

Use this quality for protein digestion for following mass spec analysis. Additionally, you can use this quality for protein sequencing.

The Lysine residues of this quality were modified by reductive methylation and purified by chromatography afterwards. This results in a stabilization of the Lysine residues against autolysis and thus no Chymotrypsin activity is detectable. However, each pack contains a Trypsin peptide (TP) standard that can be added and is providing the masses autolytic m/z 842 and 2211 . Therefore, the TP standard can be used to calibrate the mass spec analysis and thus improves the mass accuracy. We test the specifity by degrading the oxidized B-chain of the Insulin.

Trypsin NB Premium Grade, MS approved from porcine pancreas

Use this quality for use in in-gel digestion and mass spectrometry.

The Lysine residues of this quality were modified by reductive methylation and purified by chromatography afterwards. This results in a stabilization of the Lysine residues against autolysis and thus no Chymotrypsin activity is detectable. However, each pack contains a Trypsin peptide (TP) standard that can be added and is providing the masses autolytic m/z 842 and 2211 . Therefore, the TP standard can be used to calibrate the mass spec analysis and thus improves the mass accuracy. We test the specifity by degrading the oxidized B-chain of the Insulin.

Our Trypsin NB Premium-Qualität is application tested. This means that each lot is tested by mass spectrometry.

2D Elektrophorese vor Massenspektrometrie

Trypsin MS approved

 

Use this quality for use in mass spectrometry analysis.

The Lysine residues of this quality were modified by reductive methylation and purified by chromatography afterwards. This results in a stabilization of the Lysine residues against autolysis and thus no Chymotrypsin activity is detectable.

MS approved means that each lot is tested by mass spectrometry.

Trypsin 1:250 from bovine pancreas

The Serine protease mixture for cell culture contains Chymotrypsin- and non-proteolytic activities. Therefore, Trypsin 1:250 is a special enzyme mixture to achieve maximum cell viability with gentle detachment.

Use a working concentration of 250 mg/100ml, pH 7-8.

Trypsin NB Cell Culture Grade, 0.05 % solution with EDTA

Use the ready-to-use mixture for both efficient and gentle detachment od adherent cells from the surface of cell culture dishes or bottles and tissue digestion. The clear and colorless solution (in PBS) is 0.2µm filtered and therefore ready-to-use. Effective detachment and high cell viability could be demonstrated for many adherent cells types.

Although each lot is tested for pH, osmolality and sterility, we also guarantee a negative result in porcine Parvovirus (PPV), Mycoplasma and endotoxin.

Overview of the proteomics qualities as video

References

Customers’ opinion regarding our Trypsin

„For several years we have been looking for a real alternative to the „sequenzing grade“ Trypsin of a well-known manufacturer. We found it: Trypsin MS approved by SERVA. It works at least as efficiently and specifically as the „gold standard“ we used before, and does not generate any significant autolytic peaks. A major plus, particularly for a mass spec core facility, is the moderate pricing.“ Dr. Stefan Baumeister, Philipps-Universität Marburg

We use Trypsin NB Premium Grade, MS approved, from porcine pancreas for enzymatic protein digestion. The enzyme is characterized by stable activity, purity and durability. Furthermore, the trypsin is easy to handle, requires very small amounts for the digestion, it dissolves efficiently in buffer and we hardly observe self-digestion.” Christiane Rewerts, Core Unit Proteomics, Max-Planck-Institut für Psychiatrie München

„In the Proteomics Core Facility, we use SERVA trypsin for sample preparation by in-gel digestion for mass spectrometry. The SERVA Trypsin convinces with its efficiency and specificity with a very low miscleavage rate.” Dr. Andreas Marquardt, Universität Konstanz

The following institutions already use our Trypsin

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