If unwanted transcriptions are present in RT-qPCR, contamination of the PCR master mixes with double-stranded DNA may be the cause. Double-strand-specific endonucleases are time-savers and decontamination of dsDNA is much easier. Recombinantly produced dsDNases neither digest ssDNA nor RNA but can still destroy transcripts during the PCR. The addition of DTT and a brief heat treatment at 58 ˚C prior to PCR can irreversibly inactivate the heat-labile dsDNase from SERVA.
Further information at: SERVA dsDNase